Regulation and control method of CARP gene in PGC1alpha pathway, anti-doxorubicin cardiotoxicity drug and preparation method of anti-doxorubicin cardiotoxicity drug

• Main Authors: WANG XIAOXIA, CHENG YISEN, LIANG CHUNMEI, CHEN RILING, MA GUODA, LUO FEI, WANG YAJUN, XU XUSAN
• Format: Patent
• Language: Chinese; English
• Published: 30.06.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention provides a regulation and control method of a CARP gene in a PGC1 alpha pathway, an anti-adriamycin cardiotoxicity drug and a preparation method of the anti-adriamycin cardiotoxicity drug, the regulation and control method is used for the preparation process of the anti-adriamycin cardiotoxicity drug, and the regulation and control method comprises the following steps: S1, overexpressing CARP through lentivirus, activating the activity of a PGC1 alpha gene promoter, and activating the activity of the PGC1 alpha gene promoter; the expression of PGC1 alpha mRNA (messenger Ribonucleic Acid) and protein in H9c2 myocardial cells is increased; s2, CARP expression is interfered through RNAi, luciferase activity is inhibited, and expression of PGC1 alpha mRNA and protein in H9c2 myocardial cells is reduced. The CARP expression level is increased in adriamycin-induced myocardial damage, mitochondrial damage and oxidative stress can be relieved by activating a PGC1 alpha signal channel, and then myocardia