Deaminase mutant and base editor constructed based on deaminase mutant

• Main Authors: ZHANG SHUQIAN, CHEN JINLONG, CHENG TIANLIN, QIU JIAYI
• Format: Patent
• Language: Chinese; English
• Published: 30.06.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention belongs to the technical field of gene editing, and relates to a deaminase mutant and a base editor constructed based on the deaminase mutant. A novel cytidine deaminase mutant is developed, the cytidine deaminase mutant is fused to different sites of mutant nuclease represented by SpCas9, and the obtained novel fusion protein can achieve effective C-T base mutation on cytosine located at different positions of a front spacer sequence. The Cas-independent DNA off-target effect of the fusion protein obtained by the method is close to the background level, and the fusion protein has diversified editing activity windows. The base editor has a very low-level DNA/RNA off-target effect, active windows are diversified, C-T single base replacement which is wider in range, finer and higher in safety can be achieved, the application of a single base editing tool can be effectively widened, and the base editor has very high application value. 本发明属于基因编辑技术领域，涉及脱氨酶突变体及基于脱氨酶突变体构建的碱基编辑器。本发明开发了新的胞苷脱氨酶突变体，通过将胞苷脱氨