Amplification method of NK cells

The invention relates to an amplification method of NK (Natural Killer) cells. The CD16 and the trastuzumab used in the invention can rapidly and effectively stimulate the NK cells by signals, and the anti-cancer drug Kretin is used as a cell stimulating factor to activate the NK cells and promote t...

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Bibliographic Details
Main Authors FANDRIDGE FRED, HU JIANJIE, XU ZI'EN
Format Patent
LanguageChinese
English
Published 30.06.2023
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention relates to an amplification method of NK (Natural Killer) cells. The CD16 and the trastuzumab used in the invention can rapidly and effectively stimulate the NK cells by signals, and the anti-cancer drug Kretin is used as a cell stimulating factor to activate the NK cells and promote the synthesis of cell factors, so that the killing toxicity of the NK cells is effectively improved, a plurality of activation and amplification signal protein molecules required by the growth of the NK cells are provided in a culture solution, and the NK cells can be effectively killed. The amplification efficiency of NK cells in mononuclear cells of different sources is improved; in the culture process, the NK cells are firstly cultured in a coated culture bottle and then are cultured in a cell culture bag, so that the NK cells can be fully activated, apoptosis induced by a high-concentration antibody to the cells is avoided, and the amplification efficiency is ensured. The whole culture process is easy to control
Bibliography:Application Number: CN202310351592