Three-function probe based on photo-crosslinking group as well as preparation method and application of three-function probe

The invention belongs to the technical field of probes, and provides a three-function probe based on a photo-crosslinking group as well as a preparation method and application of the three-function probe. The method comprises the following steps: under a protective atmosphere, mixing a compound 1, a...

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Bibliographic Details
Main Authors LUO HUIXIN, DAI JIANYE, XI BOTING, MA XIAODONG
Format Patent
LanguageChinese
English
Published 30.06.2023
Subjects
CHEMISTRY
HETEROCYCLIC COMPOUNDS
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
METALLURGY
ORGANIC CHEMISTRY
PHYSICS
TESTING
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Summary:The invention belongs to the technical field of probes, and provides a three-function probe based on a photo-crosslinking group as well as a preparation method and application of the three-function probe. The method comprises the following steps: under a protective atmosphere, mixing a compound 1, a compound 2, 1-hydroxybenzotriazole, dimethylformamide, carbodiimide and N, N-diisopropylethylamine, and carrying out a condensation reaction to obtain the three-function probe based on the photo-crosslinking group. The probe provided by the invention can be bonded with baicalein, TFAE and baical skullcap root drug-containing serum to form a corresponding probe, and the probe competes with an original compound. Under excitation of different ultraviolet light wavelengths, the probe can realize crosslinking with small molecules and target proteins in traditional Chinese medicines, accurately reflect the marking level of a reaction group on the target proteins in a proteome through a reporter group, and selectively en
Bibliography:Application Number: CN202310239072