SNP (Single Nucleotide Polymorphism) marker related to protein content character of millet grains as well as detection primer and application of SNP marker

• Main Authors: HUANG RUI, LIU XIN, WANG HAIGANG, LIU HUAN, MU ZHIXIN, QIN HUIBIN, CHEN HONGYUN
• Format: Patent
• Language: Chinese; English
• Published: 27.06.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses an SNP (Single Nucleotide Polymorphism) marker related to the protein content character of millet grains as well as a detection primer and application of the SNP marker, the SNP marker is a GPC-09-404 marker and is positioned at the 51549836th site of the ninth chromosome of millet, and the nucleotide sequence of the SNP marker is as shown in SEQ ID NO.1 or SEQ ID NO.2. The SNP marker provided by the invention verifies that the phenotype selection efficiency of the developed SNP marker reaches 97.9% through foxtail millet natural population phenotype identification, and the protein content of foxtail millet grains can be rapidly and accurately detected. 本发明公开了一种与谷子籽粒蛋白质含量性状相关的SNP标记及其检测引物和应用，SNP标记为GPC-09-404标记，位于谷子第9染色体第51549836位，其核苷酸序列如SEQ ID NO.1或SEQ ID NO.2所示。本发明提供的SNP标记，通过谷子自然群体表型鉴定，验证开发的SNP标记表型选择效率达到97.9％，可以快速、精准地检测谷子籽粒蛋白质含量的高低。