Cationic neurotoxins

• Main Authors: ANDERSON DINA BRADY, HACKETT GAVIN STEPHEN, LIU SAI MAN
• Format: Patent
• Language: Chinese; English
• Published: 27.06.2023
• Subjects: CHEMISTRY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPECIFIC USE OF COSMETICS OR SIMILAR TOILETPREPARATIONS

The present invention relates to cationic neurotoxins. Specifically, the invention provides an engineered clostridial toxin comprising at least one amino acid modification wherein the at least one amino acid modification increases the isoelectric point (pI) of the engineered clostridial toxin to a value that is at least 0.2 pI units higher than the pI of an otherwise identical clostridial toxin lacking the at least one amino acid modification, and wherein the at least one amino acid modification is not located in the binding domain (HC domain) of the clostridial toxin. Medical uses of the engineered clostridial toxins are also provided. 本发明涉及阳离子神经毒素。具体而言，本发明提供了包含至少一个氨基酸修饰的工程化的梭菌毒素，其中所述的至少一个氨基酸修饰把工程化的梭菌毒素的等电点(pI)增大到一个值，该值比缺乏所述的至少一个氨基酸修饰、其它方面相同的梭菌毒素的pI高至少0.2个pI单位，并且其中所述的至少一个氨基酸修饰不位于梭菌毒素的结合结构域(HC结构域)。也提供了工程化的梭菌毒素的医疗用途。