SNP (Single Nucleotide Polymorphism) marker combination, primer combination and molecular identity card for sweet osmanthus variety identification

• Main Authors: LIN NAN, LI SHUQING, GUO PENG, WANG YIHAN, WEI JIANFEN, SHANG FUDE
• Format: Patent
• Language: Chinese; English
• Published: 23.06.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTEDFOR SPECIFIC APPLICATION FIELDS; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses an SNP (Single Nucleotide Polymorphism) marker combination, a primer combination and a molecular identity card for sweet osmanthus variety identification, and relates to the technical field of molecular markers. According to the invention, 14 SNP markers are screened based on high-throughput re-sequencing data in combination with methods such as bioinformatics and the like. The set of marker primer combination is used for KASP typing, and 89 parts of osmanthus fragrans varieties covering four variety groups can be completely distinguished. The application of the set of labeled primer combination to establishment of the osmanthus fragrans SNP molecular identity card can effectively improve the detection efficiency of the purity and authenticity of the osmanthus fragrans variety. Meanwhile, the genotypes of the osmanthus fragrans varieties at 14 sites are subjected to barcode expression by using a barcode generator, so that convenience is provided for rapid and intelligent query of the i