Compositions and methods for capturing target nucleic acids

• Main Authors: CAVALLO PINTO MIGUEL ANGEL, SHAH ANKUR H
• Format: Patent
• Language: Chinese; English
• Published: 23.06.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

Capture mixtures and activated capture mixtures for nucleic acid isolation and purification are provided. The mixture includes lithium lauryl sulfate, lithium hydroxide, a zwitterionic sulfonic acid buffer, and optionally protease K, a capture probe including a first specific binding partner (SBP), and a second specific binding partner immobilized on a solid support. Related combinations, methods, uses, and kits are also provided. 提供了用于核酸分离和纯化的捕获混合物和活化的捕获混合物。所述混合物包括月桂基硫酸锂、氢氧化锂、两性离子磺酸缓冲剂，以及任选地蛋白酶K、包括第一特异性结合配偶体(SBP)的捕获探针和固定于固体载体上的第二特异性结合配偶体。还提供了相关的组合、方法、用途和试剂盒。