Method for producing high-concentration bioethanol by using reeds

• Main Authors: LU JIE, LU BING, DUAN ZIYE, WANG NANKAI, LYU GUIFENG, LU HONGTAI, WANG ZHIGANG, DONG LUNING
• Format: Patent
• Language: Chinese; English
• Published: 23.06.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; SPIRITS; VINEGAR; WINE

The invention belongs to the field of biomass ethanol preparation, and particularly discloses a method for producing high-concentration bioethanol by using reeds, which is characterized in that the enzyme digestion rate can reach 97.13% through LHW-NH3.H2O/O2 pretreatment under the condition of low enzyme load (9FPU/g-PSs) and PEG 6000 additives. After the PS biosurfactant is added into the batch S-SSF, under the combination of PEG 6000 and rhamnolipid, the concentration of the bioethanol reaches 71.50 g/L, and the yield of the bioethanol is 78.40%. According to the LHW-NH3.H2O/O2 pretreatment method, three main components of the reed can be effectively separated, and high-value utilization is realized. Therefore, the method is a low-cost, green and efficient method for producing fermentable sugars, hemicelluloses and modified lignin. 本发明属于生物质乙醇制备领域，具体公开了一种利用芦苇生产高浓度生物乙醇的方法，通过LHW-NH3·H2O/O2预处理，在低酶负荷(9FPU/g-PSs)和PEG 6000添加剂下，酶消化率可达97.13％。在加入PS生物表面活性剂的分批S-SSF后，在PEG 6000和鼠李糖脂的组合下，生物乙醇浓度达到71.50g/L，生物乙醇产率为78.40％。这种