Cordyceps militaris-dictyophora indusiata mycoplasm as well as preparation method and application thereof

• Main Authors: SU MINGLI, LI JIANMEI, CHAI LINSHAN, XIE CUNYI, ZHU WANQIN, ZHANG SHUYU, ZHANG HANTIAN, WANG JIANMIN, CHEN FEI, HUAN MINGHUI
• Format: Patent
• Language: Chinese; English
• Published: 23.06.2023
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPSOR SEAWEED; CULTURE MEDIA; ENZYMOLOGY; FISHING; FOODS OR FOODSTUFFS; FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J; FORESTRY; HORTICULTURE; HUMAN NECESSITIES; HUNTING; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPECIFIC USE OF COSMETICS OR SIMILAR TOILETPREPARATIONS; SPIRITS; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES; TRAPPING; VINEGAR; WATERING; WINE

The invention provides a preparation method of cordyceps militaris-dictyophora indusiata mycoplasm, and belongs to the field of microbial fermentation. Comprising the following steps: (1) inoculating a cordyceps militaris slant strain into a liquid culture medium in a sterile state, and culturing to obtain a cordyceps militaris bacterial liquid; and (2) preparing a suspension from dictyophora indusiata and a carbon source, inoculating the cordyceps sinensis bacterial liquid into the suspension, and carrying out bidirectional fermentation culture to obtain the cordyceps sinensis-dictyophora indusiata mycoplasm. According to the method, cordyceps militaris is taken as an initial strain, fresh fungus supports in dictyophora rubrovolvata cultivation waste are taken as a main fermentation substrate, and the cordyceps militaris-dictyophora rubrovolvata characteristic mycoplasm is obtained by utilizing a liquid fermentation technology, so that the dictyophora rubrovolvata cultivation waste fungus supports are subjec