Method for tissue culture and rapid propagation by utilizing pandan leaves

• Main Authors: GUO LIXIN, HUANG TIANDAI, DENG YUTING, YANG XIUZHU, DAI XUEMEI, PENG SUNA, GU XIAOCHUAN
• Format: Patent
• Language: Chinese; English
• Published: 23.06.2023
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; TRAPPING

The invention provides a method for inducing adventitious buds by using pandan leaves so as to realize rapid proliferation, and relates to the field of plant tissue culture, and the method specifically comprises the following steps: selecting tender leaves of aseptic seedlings of pandan as explants, putting the explants into an adventitious bud induction culture medium, and after the adventitious buds are formed, transferring the explants into an elongation culture medium for culture so as to obtain the adventitious buds. Cutting elongated adventitious buds, and inoculating the adventitious buds to a rooting culture medium for rooting. According to the method, after the tender stem explants are collected from the field and induced to obtain the sterile placenta seedlings, the leaves of the sterile seedlings are utilized to induce generation, elongation and rooting of adventitious buds, and finally complete plants are obtained, so that the repeated step of collecting the explants in the field is reduced, and t