Method and device for adjusting parameters of hybridization reaction instrument

• Main Authors: WENG DANRONG, CAI SHUHENG, ZHANG WEI, ZHENG ZHENQIN, LIU HANXU
• Format: Patent
• Language: Chinese; English
• Published: 13.06.2023
• Subjects: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; BEER; BIOCHEMISTRY; CALCULATING; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPUTING; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; COUNTING; ELECTRIC DIGITAL DATA PROCESSING; ENZYMOLOGY; IMAGE DATA PROCESSING OR GENERATION, IN GENERAL; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The embodiment of the invention provides a method and device for adjusting parameters of a hybridization reaction instrument, and relates to the technical field of nucleic acid molecule hybridization. The method for adjusting the parameters of the hybridization reaction instrument comprises the following steps: acquiring surface temperature information of a biochip in the hybridization reaction instrument; obtaining a surface image of the biochip and carrying out binarization processing to obtain chip image information; processing the surface temperature information and the chip image information according to a preset database and a preset AI algorithm to obtain a chip feedback result; the feedback result is sent to the hybridization reaction instrument, the hybridization reaction instrument adjusts preset parameters according to the feedback result, and the preset parameters comprise one or more of the hybridization temperature, the hybridization time, the hybridization membrane surface state and the biochip