Acetylcholinesterase activity detection method

• Main Authors: XU JIAN, CUI LINGMIN, LAI FULI, HAO GUIFANG, LI WEIQIN, WANG ZHE
• Format: Patent
• Language: Chinese; English
• Published: 09.06.2023
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention provides an acetylcholin esterase activity detection method, which comprises the following steps: mixing 140 [mu] L of a phosphate buffer solution, 10 [mu] L of a 5, 5 '-dithiobis (2-nitrobenzoic acid) solution, 10 [mu] L of an acetylcholin esterase solution and 10 [mu] L of a sample solution in sequence, and oscillating and incubating the mixed solution; adding 10 [mu] L of a thioacetylcholine iodide solution into the mixed solution for a reaction, starting a microplate reader at the same time, and monitoring the absorbance value of a reaction system at 405 nm; and continuously monitoring for 60 minutes, and measuring the absorbance value once per minute to obtain a curve of the absorbance value and the monitoring time. The detection method disclosed by the invention can be used for more accurately measuring the change process of the activity of the acetylcholin esterase. 本发明提出了一种乙酰胆碱酯酶活性检测方法，包括如下步骤：依次将140μL磷酸盐缓冲液、10μL5,5′-二硫双(2-硝基苯甲酸)溶液、10μL乙酰胆碱酯酶溶液和10μL样品液混合，震荡孵育混合溶液；向混合溶液中加入10μL碘化硫代乙酰胆碱溶液进行反