Rapid three-dimensional microscopic imaging method and system based on wavelength multiplexing metasurface

• Main Authors: GE SUYANG, ZHANG WENFU, ZHAO WEI, WANG GUOXI
• Format: Patent
• Language: Chinese; English
• Published: 06.06.2023
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a rapid three-dimensional microscopic imaging method and system based on a wavelength multiplexing metasurface. The system comprises a light source module, a light modulation module, a sample objective table and a light collection module which are arranged along a light beam propagation direction, the light modulation module modulates the dual-wavelength light beam into illumination light with a sine stripe distribution structure and uniform illumination light with a flat phase, which are required by HiLo microscopic imaging, and projects the illumination light to the sample objective table; the light collection module realizes sample image acquisition and data transmission on different axial planes; and combining the plurality of optical slice images according to a HiLo fusion algorithm to form a three-dimensional microscopic image. According to the invention, snapshot HiLo optical section microscopic imaging is realized, and the imaging speed and stability are improved; the three-dim