Method for remarkably promoting soluble expression of expansive protein TgS and application thereof

The invention discloses a method for remarkably promoting soluble expression of expansive protein TgS and application of the method. The soluble expression of the expansive protein TgS is improved by utilizing the hydrotropy tag protein and changing and optimizing the fermentation conditions in the...

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Bibliographic Details
Main Authors SHEN QIRONG, PEI RONGHUA, KONG ZHIJIAN, LIU DONGYANG, CHEN CHEN
Format Patent
LanguageChinese
English
Published 06.06.2023
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention discloses a method for remarkably promoting soluble expression of expansive protein TgS and application of the method. The soluble expression of the expansive protein TgS is improved by utilizing the hydrotropy tag protein and changing and optimizing the fermentation conditions in the protein expression process. Based on an earlier-stage research result and current soluble protein utilization optimization requirements, it is found that in the protein expression process of an escherichia coli system, soluble expression of the expansive protein TgS can be achieved under the conditions that TRX tag protein is at the low temperature (12 DEG C), the ventilation capacity is 0.5 (V/V.min <-1 >) and 0.02 g.L <-1 > ZnSO4 and 0.1% glycerol are added. The protein fermentation process is combined and optimized from the inclusion body generation characteristics, soluble efficient expression during heterologous expression of the expansive protein TgS is achieved, the biological activity of the expansive prote
Bibliography:Application Number: CN202211622044