Hepatitis B virus nucleic acid extraction composition as well as preparation method and application thereof

• Main Authors: CHU XUNTAO, LIN YUMIAO, GOU HONGNA
• Format: Patent
• Language: Chinese; English
• Published: 06.06.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention provides a hepatitis B virus nucleic acid extraction composition and a preparation method and application thereof, and relates to the technical field of biology, the composition comprises a lysis buffer, a phase change nucleic acid protein separating agent and a phase change solid phase carrier; the phase change nucleic acid protein separating agent is a nucleic acid protein separating agent packaged by a second fusible material, and the lysis solution and the phase change nucleic acid protein separating agent are separated by a first fusible material; the phase-change solid-phase carrier is a solid-phase carrier encapsulated by a third fusible material, and the lysate and the phase-change solid-phase carrier are separated by a first fusible material; the melting point of the first fusible material is T1, the melting point of the second fusible material is T2, and the melting point of the third fusible material is T3; t1 < T2 and T1 < T3, or T1 = T2 < T3, or T1 = T3 < T2. The hepatitis B virus n