Methods of cell selection and modification of cell metabolism

• Main Authors: JAQUES CHRISTIAN M, SINGH GURPREET, BUDGE JOHN, SMALES CHRISTOPHER M, LARCHER ANDREW J, YANG RONGZHEN, ROBL JOHANNES
• Format: Patent
• Language: Chinese; English
• Published: 06.06.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

Described herein are methods and compositions for identifying, selecting, or culturing cells comprising a nucleic acid sequence of a subject of interest. In general, a nucleic acid comprising a sequence encoding an enzyme molecule involving amino acid biosynthesis and a subject nucleic acid is introduced into a cell. The cells are then grown on a culture medium lacking amino acids, thereby enabling the growth of the cells comprising the introduced nucleic acids. In some cases, the cells also comprise inhibitors of enzyme molecules to increase the severity of selection. 本文描述了用于鉴定、选择或培养包含感兴趣的对象核酸序列的细胞的方法和组合物。一般而言，将核酸导入细胞，所述核酸包含编码涉及氨基酸生物合成的酶分子的序列以及对象核酸。然后，细胞在缺少氨基酸的培养基上生长，从而使包含导入的核酸的细胞能够生长。在一些情况中，细胞还包含酶分子的抑制剂以增加选择的严格性。