Reagent, kit and method for detecting litopenaeus vannamei pathogens

• Main Authors: LIU YANHONG, SI LIEGANG, XU SHENGWEI, GE MINGFENG
• Format: Patent
• Language: Chinese; English
• Published: 02.06.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a reagent, a kit and a method for detecting litopenaeus vannamei pathogens, belongs to the field of microbiological detection, and provides a reagent and a kit containing the reagent. Wherein the reagent is prepared from the following components: 0.5 to 10 parts of 10 * Bst Buffer containing 80mM magnesium sulfate, 0.5 to 5 parts of 100mM magnesium sulfate, 0.5 to 5 parts of hydroxyl naphthol blue, 5 to 10 parts of 10mM dNTP premixed liquid, 2 to 8 parts of primer mixed liquid, 0.5 to 5 parts of DNA polymerase, 0.5 to 5 parts of reverse transcriptase, 0.5 to 5 parts of inhibitor and 10 to 30 parts of ultrapure water, and the primer mixed liquid comprises primers as shown in SEQ ID No.1 to SEQ ID No.16. The invention also provides a method for detecting the litopenaeus vannamei pathogens by using the kit. When the reagent, the kit and the method provided by the invention are used for detecting the litopenaeus vannamei pathogens, a result can be rapidly and accurately obtained, and rapid