Construction and application of plasmid expression vector based on Tac promoter
The invention relates to a transformation and construction method of a plasmid expression vector based on a Tac promoter, in which an SD sequence of the plasmid expression vector is replaced and a tag sequence is further knocked out, and the constructed expression vector is applied to recombinant fo...
Saved in:
Main Authors | , , , , , , , , , , |
---|---|
Format | Patent |
Language | Chinese English |
Published |
02.06.2023
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | The invention relates to a transformation and construction method of a plasmid expression vector based on a Tac promoter, in which an SD sequence of the plasmid expression vector is replaced and a tag sequence is further knocked out, and the constructed expression vector is applied to recombinant foreign protein expression. The invention particularly relates to human papilloma virus L1 antigen protein expression and application of an L1 antigen protein expression product in cervical cancer prevention.
本发明涉及一种基于Tac启动子的质粒表达载体改造和构建方法,其中替换其SD序列并进一步敲除标签序列,如此构建的表达载体在重组外源蛋白表达中的应用,特别是在人乳头瘤病毒L1抗原蛋白表达及其L1抗原蛋白表达产物在预防宫颈癌中的用途。 |
---|---|
Bibliography: | Application Number: CN202310114443 |