Construction and application of plasmid expression vector based on Tac promoter

• Main Authors: WANG JIANYING, ZHANG HAIJIANG, GAO WENSHUANG, ZHANG RUIXIA, SHEN ERCUI, JIANG XULIN, CHEN XIAO, LIU YONGJIANG, WU SHUMING, WANG XUEHONG, XUE JUNLIAN
• Format: Patent
• Language: Chinese; English
• Published: 02.06.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention relates to a transformation and construction method of a plasmid expression vector based on a Tac promoter, in which an SD sequence of the plasmid expression vector is replaced and a tag sequence is further knocked out, and the constructed expression vector is applied to recombinant foreign protein expression. The invention particularly relates to human papilloma virus L1 antigen protein expression and application of an L1 antigen protein expression product in cervical cancer prevention. 本发明涉及一种基于Tac启动子的质粒表达载体改造和构建方法，其中替换其SD序列并进一步敲除标签序列，如此构建的表达载体在重组外源蛋白表达中的应用，特别是在人乳头瘤病毒L1抗原蛋白表达及其L1抗原蛋白表达产物在预防宫颈癌中的用途。