Hepatitis B vaccine and production method thereof

• Main Authors: BAI XUN, XUE CHENBAO, ZHAO RAN, ZOU YENING, HU YALING, DONG GAOFENG, GAO QIANG, MENG FANHONG, ZHANG LIZHI, LI YAN, YIN WEIDONG
• Format: Patent
• Language: Chinese; English
• Published: 02.06.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention relates to a method for inducing expression of a hepatitis B surface antigen, a method for purifying the hepatitis B surface antigen, a method for producing a hepatitis B vaccine and the hepatitis B vaccine. The method for inducing expression of the hepatitis B surface antigen comprises the following steps: performing three-stage fermentation on yeast for expressing the hepatitis B surface antigen until OD600 is 100-200, adding methanol, inducing expression of the hepatitis B surface antigen, and harvesting a fermentation product. The method for purifying the hepatitis B surface antigen comprises the following steps: preliminarily purifying a fermentation product by adopting PEG (Polyethylene Glycol) sedimentation and silica gel adsorption to obtain a crude pure solution with less impure proteins, further purifying through anion exchange and gel filtration chromatography to obtain an antigen extract, and adsorbing in situ through an aluminum potassium sulfate solution to obtain the hepatitis B v