Hepatitis B vaccine and production method thereof
The invention relates to a method for inducing expression of a hepatitis B surface antigen, a method for purifying the hepatitis B surface antigen, a method for producing a hepatitis B vaccine and the hepatitis B vaccine. The method for inducing expression of the hepatitis B surface antigen comprise...
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Main Authors | , , , , , , , , , , |
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Format | Patent |
Language | Chinese English |
Published |
02.06.2023
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Subjects | |
Online Access | Get full text |
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Summary: | The invention relates to a method for inducing expression of a hepatitis B surface antigen, a method for purifying the hepatitis B surface antigen, a method for producing a hepatitis B vaccine and the hepatitis B vaccine. The method for inducing expression of the hepatitis B surface antigen comprises the following steps: performing three-stage fermentation on yeast for expressing the hepatitis B surface antigen until OD600 is 100-200, adding methanol, inducing expression of the hepatitis B surface antigen, and harvesting a fermentation product. The method for purifying the hepatitis B surface antigen comprises the following steps: preliminarily purifying a fermentation product by adopting PEG (Polyethylene Glycol) sedimentation and silica gel adsorption to obtain a crude pure solution with less impure proteins, further purifying through anion exchange and gel filtration chromatography to obtain an antigen extract, and adsorbing in situ through an aluminum potassium sulfate solution to obtain the hepatitis B v |
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Bibliography: | Application Number: CN202310082311 |