Detection method and application of related substances of pezopanib hydrochloride intermediate PZP-M1

• Main Authors: CHAI QINGYE, WANG HONGFANG, ZHANG HUI, SONG SULI, YAO KAI
• Format: Patent
• Language: Chinese; English
• Published: 30.05.2023
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention relates to the technical field of chemical drug analysis methods, in particular to a detection method and application of related substances of a pezopanib hydrochloride intermediate PZP-M1. The detection method comprises the following steps: detecting a test solution by adopting high performance liquid chromatography; detection conditions of the high performance liquid chromatography are as follows: a chromatographic column is an octadecyl silane bonded silica gel chromatographic column; the detection wavelength is 213 to 217 nm; a 0.009-0.011 mol/L aqueous solution of monopotassium phosphate with a pH value of 3.0-3.2 is used as a mobile phase A, and methanol is used as a mobile phase B for gradient elution. Through high performance liquid chromatography detection conditions, the eight related substances can be efficiently separated out at a time, and on the basis that efficient separation of the related substances and effective components is guaranteed, the method has good specificity, repeata