Establishment method and application of non-alcoholic fatty liver disease zebrafish model

• Main Authors: LIU CHENGJIE, YE DING, SUN YONGHUA
• Format: Patent
• Language: Chinese; English
• Published: 30.05.2023
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CARE OF BIRDS, FISHES, INSECTS; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW BREEDS OF ANIMALS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; SPIRITS; TRAPPING; VINEGAR; WINE

The invention relates to a method for establishing a non-alcoholic fatty liver disease zebra fish model, the method adopts CRISPR/Cas9 gene editing technology to mutate the elovl2 gene of zebra fish, and the non-alcoholic fatty liver disease model copied by the obtained zebra fish model has good similarity with human diseases. And an occurrence mechanism with similar human disease characteristics can be more accurately induced. The model is helpful for understanding the occurrence and development law of the non-alcoholic fatty liver disease, and provides a powerful basis for promoting the development of the medical treatment cause of the non-alcoholic fatty liver disease. 本发明涉及一种非酒精性脂肪肝病斑马鱼模型的建立方法，该方法采用CRISPR/Cas9基因编辑技术对斑马鱼的elovl2基因进行突变，所获得的斑马鱼模型复制的非酒精性脂肪肝病疾病模型与人类疾病相似性好，能够更准确地诱发出具有类似人类疾病特征的发生机制。该模型有助于认识非酒精性脂肪肝病的发生发展规律，为推动非酒精性脂肪肝病医疗事业的发展提供有力基础。