Method for creating transgenic Chinese rose containing RcPFARl

• Main Authors: WANG KEJIAN, LI YUNXIAN, ZHOU SHIPING, YANG FAZHONG
• Format: Patent
• Language: Chinese; English
• Published: 30.05.2023
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention provides a creation method of transgenic Chinese rose containing RcPFARl, and relates to the technical field of molecular breeding of resistant plants. The creation method of the transgenic Chinese rose containing the RcPFARl specifically comprises the following steps: S1, gene cloning; s2, constructing a carrier; s3, preparing positive cloned agrobacterium; s4, obtaining sterile seedlings of the Chinese roses; s5, inducing somatic embryos of the Chinese roses; s6, genetic transformation; and S7, obtaining a transgenic Chinese rose plant. According to the creation method of the transgenic Chinese rose containing the RcPFARl, an expression vector of a gene is constructed, and the gene is transferred into a Chinese rose plant through the mediation effect of agrobacterium, so that the gene can be used for regulating and controlling the synthesis of the palmityl alcohol in the Chinese rose plant to generate the palmityl alcohol, and the transgenic Chinese rose containing the RcPFARl is obtained. The