Preparation method of NK cells

The invention relates to a preparation method of NK cells. According to the technical scheme, the preparation of the NK cells is completed through the steps of coating a culture bottle, optimizing peripheral blood collection, and improving and promoting separation, inoculation, amplification and cel...

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Bibliographic Details
Main Authors FANDRIDGE FRED, HU JIANJIE, XU ZI'EN
Format Patent
LanguageChinese
English
Published 30.05.2023
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention relates to a preparation method of NK cells. According to the technical scheme, the preparation of the NK cells is completed through the steps of coating a culture bottle, optimizing peripheral blood collection, and improving and promoting separation, inoculation, amplification and cell harvesting of mononuclear cells. The method has the beneficial effects that the NK cells are activated and amplified by adopting mononuclear cells separated from autologous peripheral blood or cord blood cells through in-vitro operation, the aim of killing tumor cells is fulfilled by activating in-vivo specific immune response, the NK cells are cultured by adopting a pure factor method, the operation process is simple and convenient, trophoblasts are not contained, and the method is suitable for large-scale production. The preparation purity and activity of the NK cells are effectively improved, the technical cost is low, and large-scale clinical application can be carried out. 本发明涉及一种NK细胞的制备方法。采用的技术方案是：通过培养瓶包被、优
Bibliography:Application Number: CN202310190554