Primer pair and kit for identification or auxiliary identification of watermelon pulp color and application

• Main Authors: LIU JINQIU, XU JIAN, ZHANG MAN, WANG YINGYING, XU JINHUA, YU YONGHAO, YAO XIEFENG, YANG XINGPING, LOU LINA, LIU GUANG
• Format: Patent
• Language: Chinese; English
• Published: 26.05.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention belongs to the technical field of molecular marker assisted breeding, and particularly relates to a primer pair and a kit for identification or assisted identification of watermelon pulp color and application. The invention provides a primer pair for identification or auxiliary identification of watermelon pulp color. The primer pair comprises an upstream primer with a nucleotide sequence as shown in SEQ ID No.2 and a downstream primer with a nucleotide sequence as shown in SEQ ID No.3. By adopting the primer pair designed according to the InDel-P0152 molecular marker provided by the invention, the color character of the watermelon pink pulp can be accurately and quickly identified. 本发明属于分子标记辅助育种技术领域，具体涉及一种鉴定或者辅助鉴定西瓜果肉颜色的引物对和试剂盒及应用。本发明提供了一种鉴定或者辅助鉴定西瓜果肉颜色的引物对，所述引物对包括核苷酸序列如SEQ ID No.2所示的上游引物和核苷酸序列如SEQ ID No.3所示的下游引物。采用本发明提供的根据InDel-P0152分子标记设计的引物对，可以准确快速的鉴定西瓜粉色果肉颜色性状。