External tissue culture method of artemisia apiacea stems

The invention discloses an artemisia apiacea stem external tissue culture method in the field of external tissue culture, and the method comprises the following steps: step 1, preparing a culture medium, taking a 1/2 MS culture medium as a basic culture medium, and adding raw materials NAA, Huabao N...

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Bibliographic Details
Main Authors WEI SHUNI, XIE GANG, LIANG YUNMEI, WANG MENGCHU, PAN YUSHU
Format Patent
LanguageChinese
English
Published 26.05.2023
Subjects
AGRICULTURE
ANIMAL HUSBANDRY
FISHING
FORESTRY
HUMAN NECESSITIES
HUNTING
NEW PLANTS OR PROCESSES FOR OBTAINING THEM
PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
TRAPPING
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Summary:The invention discloses an artemisia apiacea stem external tissue culture method in the field of external tissue culture, and the method comprises the following steps: step 1, preparing a culture medium, taking a 1/2 MS culture medium as a basic culture medium, and adding raw materials NAA, Huabao No.1, white granulated sugar, agar and potatoes; step 2, selecting an artemisia apiacea explant and shearing artemisia apiacea stems and leaves on the artemisia apiacea explant; step 3, slicing the sweet wormwood stems and leaves to prepare explants, and inoculating the explants; step 4, culturing the explant under the culture conditions that the temperature is 24-28 DEG C, the illumination time is 14 hours, and the illumination intensity is 2000-4000 Lux; 5, after tender shoots on the explants grow high, the explants are transferred and propagated. In the first step, the content of NAA is 0.5-0.7 mg/l, the content of Huabao No.1 is 0.5-1.0 g, the content of white granulated sugar is 25-35 g, the content of agar is
Bibliography:Application Number: CN202211708262