Chaetomium olivum histone deacetylase as well as coding gene and application thereof

• Main Authors: ZHANG LIXIN, XIA XUEKUI, WANG JIAHUI, CHEN QIAONI, CAO SHENGLING, ZHAO PEIPEI, WANG MENGMENG, YIN XIN, LIU HAIRONG, MENG YIWEI, LIN JIAYING
• Format: Patent
• Language: Chinese; English
• Published: 23.05.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention belongs to the technical field of microorganisms and biological medicines, and particularly relates to olive chaetomium globosum histone deacetylase as well as a coding gene and application of the olive chaetomium globosum histone deacetylase. According to the present invention, the gene knockout is performed on the HDAC in the fungus strain Chaetomium olivum SD-80A, such that the activation of the recessive biosynthesis gene cluster is achieved, and the yield of the compound Cochlidinol is increased; researches find that the compound shows good anti-microbial, anti-inflammatory, anti-coagulation and anti-cancer activities, and has the potential of being developed into a drug lead compound, so that the compound has good practical application value. 本发明属于微生物和生物医药技术领域，具体涉及一种橄榄色毛壳组蛋白去乙酰化酶及其编码基因和应用。本发明对真菌菌株橄榄色毛壳(Chaetomium olivaceum)SD-80A中的HDAC进行基因敲除，实现了对隐性生物合成基因簇的激活并且提高了化合物可皆霉素(Cochlidinol)的产量。研究发现，该化合物显示出良好的抗微生物、抗炎、抗凝血以及抗癌活性，具备开发为药物先导化合物的潜力，因此具有良好的实际应用之价值。