Carbonic anhydrase artificial enzyme as well as preparation method and application thereof

• Main Authors: ZHAO CHANGSHENG, CHENG CHONG, LI LIN, LI SHUANG, XU WENJIE, SUN SHUDONG, WANG MAO
• Format: Patent
• Language: Chinese; English
• Published: 23.05.2023
• Subjects: ACYCLIC OR CARBOCYCLIC COMPOUNDS; CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY; CHEMISTRY; COMPOSITIONS BASED THEREON; COMPOUNDS THEREOF; INORGANIC CHEMISTRY; MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONSONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS; METALLURGY; NON-METALLIC ELEMENTS; ORGANIC CHEMISTRY; ORGANIC MACROMOLECULAR COMPOUNDS; PERFORMING OPERATIONS; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL; SEPARATION; THEIR PREPARATION OR CHEMICAL WORKING-UP; THEIR RELEVANT APPARATUS; TRANSPORTING

The invention relates to a carbonic anhydrase artificial enzyme as well as a preparation method and application thereof, and belongs to the field of preparation of artificial enzymes. The invention provides a carbonic anhydrase artificial enzyme. The artificial enzyme is prepared from a ligand and a zinc salt through a hydrothermal method, wherein the ligand is selected from the group consisting of 1, 4-bis (1H-pyrazol-4-yl) benzene, 1, 3-bis (1H-pyrazol-4-yl) benzene, 2, 7-bis (1H-pyrazol-4-yl) naphthalene, 2, 5-bis (1H-pyrazol-4-yl) pyridine or 2, 7-bis (1H-pyrazol-4-yl) pyrene. The artificial enzyme obtained in the invention has high CA enzyme simulation activity, shows excellent CA-like reaction kinetics (Vmax: 3.83 M s <-1 >, turnover number (TON): 5.97 * 10 <-3 > s <-1 >), and has ultrafast and excellent activity in all reported simulated CA catalysts. 本发明涉及一种碳酸酐酶人造酶及其制备方法和应用，属于人造酶的制备领域。本发明提供一种碳酸酐酶人造酶，所述人造酶由配体和锌盐通过水热法制得；其中，所述配体选自：1,4-二(1H-吡唑-4-基)苯，1,3-二(1H-吡唑-4-基)苯，2,7-二(1H-吡唑-4-基)萘，2,5-二(1H-吡唑-4-基)吡啶或2