Endoglucanase mutant as well as coding gene, recombinant vector, recombinant thallus and enzyme preparation thereof and application of endoglucanase mutant and coding gene, recombinant vector, recombinant thallus and enzyme preparation

• Main Authors: SHENG YIJIE, WANG MINGHUI, LI ANNI, HUANG HE, LI XIUJUAN, WEI JUNNAN, QIAO JIE
• Format: Patent
• Language: Chinese; English
• Published: 16.05.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to renewable energy sources and bioengineering technologies, and discloses an endoglucanase mutant as well as a coding gene, a recombinant vector, a recombinant thallus and an enzyme preparation thereof, and applications of the endoglucanase mutant and the coding gene, the recombinant vector, the recombinant thallus and the enzyme preparation. The endoglucanase mutant is an enzyme with an amino acid sequence as shown in SEQ ID NO. 1, and an enzyme with an amino acid sequence as shown in SEQ ID NO. The amino acid sequence is an amino acid sequence which is obtained by substituting, deleting or adding one or more amino acid residues on the 12th site and the 264th site of the amino acid as shown in SEQ ID NO.1 and still has endoglucanase mutant activity; or the amino terminal and/or the carboxyl terminal of the amino acid sequence are/is connected with a labeled enzyme shown in the amino acid sequence; or the amino terminal of the amino acid sequence is connected with an enzyme shown in the