Aspergillus aculeatus and application thereof in production of tannase and gallic acid

• Main Authors: LI BICHAN, WU QIYU, ZHANG MIN, XIE WENYI, CHEN BINGWEI, YANG HAINAN, LAI MEILING
• Format: Patent
• Language: Chinese; English
• Published: 16.05.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses aspergillus aculeatus and application thereof in production of tannase and gallic acid, and belongs to the technical field of microorganisms and tannin degradation. The microorganism is classified and named as Aspergillus aculeatus WYT2101, and is preserved in the Guangdong Microbiological Culture Collection Center, the preservation number is GDMCC No.62691, the preservation date is August 10, 2022, and the preservation address is the 5th floor, No. 59 building, Institute of Microbiology, Academy of Sciences, Guangdong. The aspergillus aculeatus can be used for preparing tannase with high enzyme activity (the activity of tannase in fermentation supernatant can reach 44.45 U/mL) and good thermal stability by utilizing the tannase gene of the aspergillus aculeatus; in addition, the pomegranate rind can be used for efficiently producing tannase (2.14 U/mL) and gallic acid (the yield reaches 1.7 mg/mL), and the pomegranate rind has the potential of being applied to industrial mass producti