Preparation method of functionalized magnetic bead with surface coupled with photoaffinity chain

• Main Authors: XIAO GUYU, YAN JIZHONG, ZHANG HUI, YE JIAMIN, YAO JIANGYU
• Format: Patent
• Language: Chinese; English
• Published: 16.05.2023
• Subjects: BASIC ELECTRIC ELEMENTS; CHEMISTRY; ELECTRICITY; HETEROCYCLIC COMPOUNDS; INDUCTANCES; MAGNETS; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; METALLURGY; NANOTECHNOLOGY; ORGANIC CHEMISTRY; PERFORMING OPERATIONS; SELECTION OF MATERIALS FOR THEIR MAGNETIC PROPERTIES; SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; TRANSFORMERS; TRANSPORTING

The invention discloses a preparation method of a functional magnetic bead coupled with a photoaffinity chain on the surface, which comprises the following steps: activating a carboxyl magnetic bead, incubating the activated carboxyl magnetic bead and a photoaffinity connecting chain to obtain a photoaffinity connecting chain loaded carboxyl magnetic bead, sealing the surface of the carboxyl magnetic bead loaded by the photoaffinity connection chain by using a sealing liquid to obtain a functionalized carboxyl magnetic bead; the photoaffinity connecting chain takes trifluoromethyl phenyl bis (aziridine) as a photosensitive group and has relatively good chemical stability, a carbene intermediate generated after photolysis can be irreversibly combined with most of small molecules and is relatively stable, a large number of different types of small molecules do not need structure-function relationship (QSAR) research data and corresponding chemical modification, and the photoaffinity connecting chain has relativ