Engineered leucine decarboxylases

• Main Authors: LIU ZHIZHONG, VALIEUX CHRISTIAN JEAN, JENNE STEPHAN, THIEDE LINE KUITINMONT, DELLAS NIKKI, DO FAYE LONG, MCCLUSKEY KRIEN
• Format: Patent
• Language: Chinese; English
• Published: 12.05.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention provides engineered leucine decarboxylase (LDC) polypeptides and compositions thereof, as well as polynucleotides encoding the engineered leucine decarboxylase (LDC) polypeptides. In some embodiments, the engineered LDC polypeptides are optimized to provide enhanced catalytic activity, as well as reduced sensitivity to proteolysis and/or increased tolerance to low pH environments. In some embodiments, the engineered LDC polypeptides are optimized to provide improved storage stability. The invention also provides methods of using the compositions comprising the engineered LDC polypeptides for therapeutic and industrial purposes. 本发明提供了工程化亮氨酸脱羧酶(LDC)多肽及其组合物、以及编码工程化亮氨酸脱羧酶(LDC)多肽的多核苷酸。在一些实施方案中，工程化LDC多肽被优化为提供增强的催化活性，以及降低的对蛋白水解的敏感性和/或增加的对低pH环境的耐受性。在一些实施方案中，工程化LDC多肽被优化为提供改进的储存稳定性。本发明还提供了使用包含工程化LDC多肽的组合物用于治疗和工业目的的方法。