Method for exploring influence of TRPV1 on lipid metabolism of type 2 diabetes

• Main Authors: KONG DANLI, WU SHUFA, TAN XIYU, YANG ZIHUA, HU WEI, CHEN XUEQIN, DONG WENJIAO, YU HAIBING, HUANG JIALU, KIM SEULYE, LING YAO, CAO RUDAI, DING YUANLIN, WANG SHIHONG
• Format: Patent
• Language: Chinese; English
• Published: 12.05.2023
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a method for exploring the influence of TRPV1 on lipid metabolism of type 2 diabetes mellitus, which comprises the following steps of: firstly, collecting data and samples, namely collecting demographic data, clinical biochemical index results and blood samples of type 2 diabetes mellitus patients and healthy control people; according to the invention, a mouse with diabetes mellitus type 2 is cultured, a mouse model with diabetes mellitus type 2 is constructed, glucose tolerance (OGTT) and insulin resistance (ITT) are fed, histopathologic changes of the mouse are detected and recorded, and a TRPV1 agonist and an inhibitor are injected into the abdominal cavity of the mouse with diabetes mellitus. A corresponding metabolic pathway of TRPV1 channel protein participating in regulation and control of fat metabolism of type 2 diabetes mellitus is detected, so that the regulation and control action mechanism of TRPV1 on fat metabolism of type 2 diabetes mellitus is determined, a scientific b