Method for synchronously detecting bone marrow transplantation chimeric rate of genetically modified mouse by qPCR (quantitative polymerase chain reaction)

• Main Authors: LIU BIN, CHEN XIJIAN, GUO JINWEI, LENG JING, XIE KAIQI, ZHOU YINGBI, GE JIAHUI, YU GANG
• Format: Patent
• Language: Chinese; English
• Published: 12.05.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to the field of bone marrow transplantation research, in particular to a method for synchronously detecting the bone marrow transplantation chimeric rate of a genetically modified mouse by qPCR (quantitative polymerase chain reaction), which comprises the following steps: A, acquiring the average number CTT of CT (computed tomography) values of target genes of sample groups (T) after bone marrow transplantation; b, acquiring a CT value CTT internal reference of the internal reference gene corresponding to the target gene of each sample group; c, subtracting the CTT internal reference from the CTT purpose to obtain delta CTT; d, obtaining the average number CTC of the CT values of the target genes of the control group (C) which is not processed; f, acquiring the CT value CTC internal reference of the internal reference genes corresponding to the target genes of the control group; g, subtracting the internal reference of the CTC from the aim of the CTC to obtain delta CTC; h, subtracting t