Application of rice OsNAC42 gene in regulation and control of grain cadmium accumulation amount, sgRNA, recombinant vector and application thereof

• Main Authors: SHEN RENFANG, LIU YUSONG, ZHU XIAOFANG, TAO YE
• Format: Patent
• Language: Chinese; English
• Published: 12.05.2023
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; ORGANIC CHEMISTRY; PEPTIDES; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention relates to the technical field of gene engineering, in particular to application of a rice OsNAC42 gene in regulation and control of grain cadmium accumulation, sgRNA, a recombinant vector and application of the sgRNA and the recombinant vector. According to the present invention, the Cd content of the grains of CRISPR mutant library rice planted in the Cd-polluted farmland is determined, the mutant osnac42 with the Cd content significantly lower than the Cd content of the wild Nipponbare nip is screened, the new mutant osnac42 with the gene function deletion is further established through the CRISPR/Cas9 gene editing technology, and the new mutant osnac42 and the wild Nipponbare nip are planted in the moderately mild Cd-polluted farmland, it is found that the Cd content of osnac42 mutant grains is obviously lower than that of wild type grains, but yield-related agronomic traits are basically not affected. The OsNAC42 gene not only can be used for perfecting a regulation and control network of r