Bacillus amyloliquefaciens genome scale metabolic network model, construction method and application

• Main Authors: WANG YUZHOU, HUANG HE, YE CHAO, QIAN JINYI, LIU XINER
• Format: Patent
• Language: Chinese; English
• Published: 09.05.2023
• Subjects: BEER; BIOCHEMISTRY; CALCULATING; CHEMISTRY; COMPOSITIONS THEREOF; COMPUTING; COUNTING; CULTURE MEDIA; ELECTRIC DIGITAL DATA PROCESSING; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTEDFOR SPECIFIC APPLICATION FIELDS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PHYSICS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a bacillus amyloliquefaciens genome scale metabolic network model, a construction method and application, and belongs to the field of system biology. The method comprises the following steps: firstly, automatically constructing a bacillus amyloliquefaciens coarse model according to a proteome sequencing result of bacillus amyloliquefaciens in a Uniport database; secondly, according to a literature database, a metabolic pathway diagram and a biochemical database, performing a synthetic reaction of manual refining and acetoin addition on the rough model, and constructing a genome scale metabolic network model; converting the genome scale metabolic network model into a computer readable mathematical model; and finally, analyzing the mathematical model by using a flux equilibrium analysis method, and comparing with an experimental value in the literature. The method has the advantages of being short in construction time, easy to operate and high in accuracy, the nutritional conditions for