Gene knockout method and application of phage reproduction related genes

• Main Authors: LI PEIZE, LI YUEYING, YANG HONGJIANG, HAN QINGZHU, LI DONGHANG
• Format: Patent
• Language: Chinese; English
• Published: 09.05.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a gene knockout method of a phage reproduction related gene, which is characterized in that the phage reproduction related gene is knocked out as a target gene, in the method, when a gene deletion mutant strain is screened, the interference of a wild type strain is eliminated by using a phage, and meanwhile, the antibiotic sensitivity is verified in an antibiotic-containing LB culture medium; eliminating the interference of a single exchanger; according to the method, gene deletion mutant strains can still be screened out after sensitive inactivation of sucrose regulated and controlled by the sacB gene in the gene knockout process. The invention provides a method for rapidly screening out a gene deletion mutant strain after sucrose sensitive inactivation regulated by a sacB gene in a gene knockout process. The gene deletion mutant strain obtained by the method disclosed by the invention can be compared with a wild strain in a biological characteristic experiment, and the interaction mo