Chimeric antigen receptor T cell targeting BCMA as well as preparation method and application of chimeric antigen receptor T cell

• Main Authors: SUN SHISHUO, ZHENG JUNNIAN, GAO XIAOGE, ZHANG QING
• Format: Patent
• Language: Chinese; English
• Published: 09.05.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention discloses a chimeric antigen receptor T cell targeting BCMA and a preparation method and application thereof.The chimeric antigen receptor T cell is a T cell expressing a BCMA specific chimeric antigen receptor, and the chimeric antigen receptor comprises an antibody capable of targeting BCMA, a hinge region, a transmembrane region, a costimulatory signal structural domain and an intracellular signal transduction structural domain; the amino acid sequence of the antibody is shown as SEQ ID NO.11. The chimeric antigen receptor T cell specifically targets BCMA positive cells, and can be used for treating BCMA positive B cell lymphoma, multiple myeloma, plasma cell leukemia and other diseases. 本发明公开了一种靶向BCMA的嵌合抗原受体T细胞及其制备方法和应用，所述嵌合抗原受体T细胞为表达BCMA特异性嵌合抗原受体的T细胞，所述嵌合抗原受体包含能够靶向BCMA的抗体、铰链区和跨膜区、共刺激信号结构域和胞内信号传导结构域，所述抗体的氨基酸序列如SEQ ID NO:11所示，本发明公开的嵌合抗原受体T细胞特异性靶向BCMA阳性细胞，可以用于治疗BCMA阳性B细胞淋巴瘤、多发性骨髓瘤和浆细胞白血病等疾病。