Molecular marker, primer group, kit and method for identifying sex of pelteobagrus vachelli and application

• Main Authors: MEI JIE, LIAO QIAN, HU JINGQI, WANG ZHONGWEI, WANG DEZHONG, GONG GAORUI, JIANG YOUBO, WU JIANKAI
• Format: Patent
• Language: Chinese; English
• Published: 05.05.2023
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CARE OF BIRDS, FISHES, INSECTS; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTEDFOR SPECIFIC APPLICATION FIELDS; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW BREEDS OF ANIMALS; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; SPIRITS; TRAPPING; VINEGAR; WINE

The invention relates to the technical field of sex detection of pelteobagrus vachelli, and discloses a molecular marker, a primer group, a kit and a method for identifying sex of pelteobagrus vachelli. The molecular marker is a differential sequence of a nucleotide sequence as shown in SEQ ID NO.1 from a Pelteobagrus vachelli X chromosome genome and a nucleotide sequence as shown in SEQ ID NO.2 from a Pelteobagrus vachelli Y chromosome genome, the differential sequence is shown in SEQ ID NO.3, and the differential sequence is a specific sequence of the Pelteobagrus vachelli Y chromosome genome. The identification primer, the kit and the method provided by the embodiment of the invention are not influenced by the individual development period and the environment, the sex identification is simple, convenient and rapid, the requirement on the sample is low, the accuracy of the identification result is high, so that the defects of relatively complicated operation, relatively long time consumption and the like of