VIGS silence vector of jujube ZjCLA gene and application of VIGS silence vector

• Main Authors: ZHANG YAO, LI SHIJIA, ZHAO JIN, NIU NAZI
• Format: Patent
• Language: Chinese; English
• Published: 05.05.2023
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention relates to the field of plant genetic engineering, in particular to a VIGS silencing vector of a jujube ZjCLA gene and application of the VIGS silencing vector. The invention provides an application of a specific fragment in construction of a VIGS silencing vector of a jujube ZjCLA gene, the specific fragment comprises a nucleotide sequence as shown in SEQ ID NO.1, and the jujube ZjCLA gene comprises a gene with a sequence number of XM048477782.1. The invention further provides a construction method of the jujube ZjCLA gene. The VIGS silencing vector constructed by using the specific fragment provided by the invention can successfully infect jujube seedlings and induce silencing of jujube endogenous ZjCLA, the expression level of the jujube ZjCLA gene is effectively reduced, a virus-induced gene silencing character is obtained, the function of the jujube ZjCLA gene can be verified, and the VIGS silencing vector has an application value in functional gene research of jujubes. In addition, the inv