Reaction liquid and kit for human CYP2D6 genotype detection

• Main Authors: LI HONGDONG, DENG BO, FAN LIANGBO, FU XIAONI, ZHU MUZI, WANG HONG, MASSIA, GUO XIAORONG
• Format: Patent
• Language: Chinese; English
• Published: 02.05.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a reaction liquid and a kit for human CYP2D6 genotype detection. The reaction liquid and the kit are used for detecting a CYP2D6 gene c.1846Ggt; a, c.100Cgt, and c. T, c, 1758Ggt; a, c, 2988Ggt, and c, the kit comprises a reaction solution for genotype detection of four SNP sites A and A, and the reaction solution comprises specific primers of the four SNP sites, a Taqman probe, an internal standard primer and probe, Taq enzyme, dNTPs and a buffer solution. By designing the concentrations of the primers of the four sites and the primer probe group, the amplification efficiency of each site and the endogenous reference gene is consistent, and the later result interpretation is facilitated. The kit provided by the invention has extremely high specificity and detection efficiency, can be completed in four reaction system closed tubes, greatly reduces the operation intensity, can obtain a result in about 1 hour, covers detection of a plurality of sites, and provides more patient gene infor