Primer group, kit and detection method for Diego blood type genotyping

• Main Authors: WANG BUQIANG, GAO HONGJUN, WU MINGLEI, CHEN YUPING, CHEN FANGFANG
• Format: Patent
• Language: Chinese; English
• Published: 02.05.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to the field of biochemical analysis and detection, in particular to a Diego blood group genotyping primer group, a kit and a detection method thereof.The Diego blood group genotyping primer group comprises a primer pair capable of amplifying a target site c.1694 in an SLC4A1 gene, a primer pair capable of amplifying the target site c.1694 in the SLC4A1 gene, a primer pair capable of amplifying the target site c.1694 in the SLC4A1 gene and a primer pair capable of amplifying the target site c.1694 in the SLC4A1 gene, the invention also provides a method for detecting the Diego blood type based on the non-therapeutic purpose, which comprises the following steps: extracting DNA in blood to be detected as a template, taking the Diego blood type genetic typing primer group as an amplification primer, carrying out real-time fluorescent PCR amplification, obtaining a melting curve of a fluorescence signal and temperature change, and judging a typing result according to the melting curve. The p