Application of SLC9A1 as target spot in inhibition of avian leukosis virus

• Main Authors: YANG QUN, ZHANG FANFAN, WU CHENGCHENG, ZENG YANBING, KANG ZHAOFENG, TAN JIA, TAN MEIFANG, LI HAIQIN
• Format: Patent
• Language: Chinese; English
• Published: 02.05.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention discloses application of SLC9A1 as a target spot in inhibition of avian leukosis viruses, replication of J subtype avian leukosis viruses can be remarkably inhibited by inhibiting or silencing DF-1 fibroblast SLC9A1 genes, and the SLC9A1 can be used as a candidate medicine for inhibiting the J subtype avian leukosis viruses. Six sgRNA sequences are designed by taking the SLC9A1 gene as a target spot, after DF-1 cells are transfected, a cell line for stably expressing the sgRNA sequences is screened out through puromycin, the SLC9A1 gene in host cells is knocked out, and the obtained monoclonal cell strain has resistance phenotype to the J subtype avian leukosis virus, can remarkably inhibit duplication of the J subtype avian leukosis virus in the DF-1 cells, and has a good application prospect. And a new direction is further provided for the research of the J subtype avian leukosis virus biological medicine. 本发明公开了SLC9A1作为靶点在抑制禽白血病病毒中的应用，通过抑制或者沉默DF-1成纤维细胞SLC9A1基因，可以显著抑制J亚型禽白血病病毒的复制，可以作为抑制J亚型禽白血病