Laccase, preparation method thereof and preparation method of magnolol

• Main Authors: CHEN XIANQING, WANG XIAO, DU QIUHUI, HUANG LIHUI, LAI CHANGLONG
• Format: Patent
• Language: Chinese; English
• Published: 02.05.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses laccase, a preparation method thereof and a preparation method of magnolol. The amino acid sequence of the laccase is shown as any one amino acid sequence in SEQ ID NO: 1-SEQ ID NO: 6, or is an amino acid sequence which is at least 90%, at least 95%, at least 96%, at least 97%, at least 98% or at least 99% similar to any one amino acid sequence in SEQ ID NO: 1-SEQ ID NO: 6. The laccase is obtained by constructing escherichia coli genetically engineered bacteria, performing induced expression on the genetically engineered bacteria and purifying. And 4-allylphenol is catalyzed by laccase to generate magnolol. The key enzyme for magnolol synthesis is obtained through identification, the purpose of producing magnolol from escherichia coli in a heterologous mode is achieved, and the method has the advantages of being high in selectivity, free of pollution and short in production period. 本申请公开了一种漆酶及其制备方法、厚朴酚的制备方法，漆酶的氨基酸序列如SEQ ID NO：1～SEQ ID NO：6中任一氨基酸序列所示，或者为与SEQ ID NO：1～SEQ ID NO：6中任一氨基酸序列至