High-throughput identification method for traditional Chinese medicine and personal care products in blood

• Main Authors: ZHU CHI, GUO JING, YU HONGXIA, QU CHANGSHENG, YAO HONGYE, WEN HAILING, ZUO WU, WANG WEIXIA, SHI WEI
• Format: Patent
• Language: Chinese; English
• Published: 28.04.2023
• Subjects: INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTEDFOR SPECIFIC APPLICATION FIELDS; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses a high-throughput recognition method for drugs and personal care products in blood, and belongs to the technical field of analytical chemistry. The method is based on a high-resolution mass spectrum, and comprises the following steps: firstly, establishing a typical PPCPs mass spectrum database which comprises names, molecular formulas, CAS numbers, adduct ion modes and primary mass spectrum peak m/z of 100 typical PPCPs and one or two characteristic fragments with the highest relative intensity in a secondary mass spectrum; secondly, performing high-efficiency separation on low-concentration PPCPs in blood by utilizing ultra-high performance liquid chromatography-high-resolution mass spectrometry after pretreatment of a blood sample, and simultaneously performing rapid and continuous scanning on primary and secondary mass spectrograms of the PPCPs in the blood in single primary analysis and determination; and finally, matching the spectrogram information with a typical PPCPs mass spec