Real-time fluorescent nucleic acid isothermal amplification detection kit for bladder cancer as well as special primer and probe thereof

• Main Authors: JU JINLIANG, ZHANG SHIQI, CUI ZHENLING, RAN PENGZHAN
• Format: Patent
• Language: Chinese; English
• Published: 28.04.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a real-time fluorescent nucleic acid isothermal amplification detection kit for bladder cancer as well as a special primer and a probe thereof, and belongs to the technical field of tumor diagnosis. The kit provided by the invention comprises reagents for respectively and specifically detecting the following detection genes based on a real-time fluorescent nucleic acid isothermal amplification method: CDC2, MDK, IGF2, UPK1B and TERT, optional HOXA13, IGFBP5, CXCR2, CRH and ANXA10, and GAPDH, PKG1, PPIA, UBC or ABL1 for detecting reference genes, the kit can adopt a real-time fluorescent nucleic acid isothermal amplification method and take random urine of a patient as a to-be-detected sample to detect bladder cancer (including preliminary diagnosis and recurrent patients) in a high-sensitivity manner, and has a relatively high negative predicted value, so that unnecessary cystoscopy of a negative object can be effectively avoided, and the detection cost can be reduced. 本发明公开一种膀胱癌的实时荧光核