Traditional Chinese medicine ointment detection method for quantitative analysis of multi-components by single marker

• Main Authors: WANG PING, MA YIPING, HUANG TIANZHUO
• Format: Patent
• Language: Chinese; English
• Published: 25.04.2023
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention relates to the technical field of traditional Chinese medicines, in particular to a traditional Chinese medicine ointment detection method for quantitative analysis of multi-components by single marker, which comprises the following steps: S1, thin-layer chromatography step and S2, high performance liquid chromatography step. According to the method, a high performance liquid chromatography is adopted for quantitative determination of a preparation, and gradient wavelength is adopted for determination of a mobile phase as follows: in 0-25 min, the concentration of a mobile phase A is increased to 10-20% from 5-10%, and the concentration of a mobile phase B is reduced to 80-90% from 90-95%; in 25-45 min, the concentration of the mobile phase A is increased to 15-30% from 10-20%, and the concentration of the mobile phase B is reduced to 75-85% from 80-90%; in 45-60 minutes, the concentration of the mobile phase A is increased to 40-60% from 15-30%, and the concentration of the mobile phase B is re