SV40LTAE1A residual DNA kit and application

• Main Authors: ZHANG TIANYANG, ZHENG MENGTAO, FAN PENGCHENG, LI ZHA, CAI YUQING, LIU LIMEI
• Format: Patent
• Language: Chinese; English
• Published: 25.04.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The invention discloses an SV40LTAamp and a method for implementing the SV40LTAamp; the E1A residual DNA kit comprises the following components: SV40LTAamp, E1A residual DNA, E1A residual DNA and E1A residual DNA, an E1A DNA quantitative reference substance, and SV40LTAamp; the kit comprises the following components: E1A Primer MIX, qPCRReaction Buffer and a DNA (Deoxyribose Nucleic Acid) diluent. According to the method, a vortex oscillator is used for oscillation for 5-10 seconds during dilution, and then rapid centrifugation is performed for 4-8 seconds at the speed of 800-1000 rpm, so that DNA fragments with the size of 150-250 bp can be fully and uniformly dispersed in a dilution tube, and the influence of winding of the DNA fragments on the reaction efficiency is reduced. In addition, SV40LTAamp is adopted; the E1A PrimerMIX accounts for 15-20% of the total volume of the qPCR reaction liquid, so that a plurality of initiation points exist in the PCR reaction, and the chain extension efficiency of the PC