Recombinant vector for preparing HLA/beta2M compound as well as preparation method and application of recombinant vector

• Main Authors: ZHANG YONGXIA, XIAO SHUIBING, LU LIANG, WAN DINGYI, QIN FUBO, DAI TENGFEI
• Format: Patent
• Language: Chinese; English
• Published: 25.04.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention discloses a recombinant vector for preparing an HLA/beta2M compound as well as a preparation method and application of the recombinant vector, and relates to the technical field of preparation of an antibody of an MHC-polypeptide compound. The invention provides a recombinant vector for preparing an HLA/beta2M compound, an FOS leucine zipper bZIP motif is inserted between NheI/AvrII restriction enzyme cutting sites of an original vector, a JUN leucine zipper bZIP motif is inserted between BsiWI/BlpI restriction enzyme cutting sites, a beta2M gene sequence is inserted between AgeI/NheI restriction enzyme cutting sites, and an HLA sequence is inserted between specific restriction enzyme cutting sites, so that the finally prepared HLA/beta2M compound has high expression quantity which is up to 92.45 mg/L. 本发明公开了一种用于制备HLA/β2M复合物的重组载体及其制备方法和应用，涉及MHC-多肽复合物的抗体的制备技术领域；本发明提供了一种用于制备HLA/β2M复合物的重组载体，在原始载体的NheI/AvrII酶切位点之间插入FOS亮氨酸拉链bZIP motif，在BsiWI/BlpI酶切位点之间插入JUN亮氨酸拉链bZIP motif，在AgeI/NheI酶切位点之间插入β2M基因序列，在特