Preparation and application of mycobacterium tuberculosis antigen composition PFHP010

• Main Authors: WAN KANGLIN, FAN XUETING, LIU HAICAN, LI MACHAO, CAO BIN, JIANG YI
• Format: Patent
• Language: Chinese; English
• Published: 25.04.2023
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; ORGANIC CHEMISTRY; PEPTIDES; PHYSICS; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; TESTING; VINEGAR; WINE

The invention relates to the technical field of genetic engineering, in particular to preparation and application of a mycobacterium tuberculosis antigen composition PFHP010. The mycobacterium tuberculosis antigen composition PFHP010 provided by the invention comprises antigens PPE18, FadD28, PlcA and HspX, and the antigens PPE18, FadD28, PlcA and HspX are fused into a novel fusion protein PFHP010f through a genetic engineering technology. The multi-component protein subunit vaccine constructed by the invention can induce an organism to generate relatively strong cell and humoral immunity, and has no obvious toxic and side effects in a mouse immunization process. A plurality of potential antigen components in different infection stages of tubercle bacillus are combined to prepare the vaccine, so that the vaccine has higher pertinence, can reach or even exceed the immune protection effect of BCG, has the advantages of low cost, good safety and the like, and is expected to become a candidate vaccine for prevent